Cover Picture: Long-Wavelength Fluorescence from 2-Aminopurine-Nucleobase Dimers in DNA (ChemPhysChem 8/2008)
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چکیده
منابع مشابه
Long-wavelength fluorescence from 2-aminopurine-nucleobase dimers in DNA.
When 2-aminopurine (2AP) is substituted for adenine in DNA, it is widely accepted that its fluorescence spectrum is essentially unchanged from that of the free fluorophore. We show that 2AP in DNA exhibits long-wavelength emission and excitation bands, in addition to the familiar short-wavelength spectra, as a result of formation of a ground-state heterodimer with an adjacent, pi-stacked, natur...
متن کاملFluorescence properties of 2-aminopurine in human telomeric DNA.
The substitution of 2-aminopurine (Ap) for A7 in the human telomeric sequence d[AGGG(TTAGGG)(3)] resulted in a significant increase in the fluorescence intensity of Ap for the conformational change from duplex to quadruplex.
متن کاملUltrafast transient-absorption and steady-state fluorescence measurements on 2-aminopurine substituted dinucleotides and 2-aminopurine substituted DNA duplexes
Ultrafast transient-absorption and steady-state fluorescence measurements have been performed on dinucleotides comprising the fluorescent adenine analogue 2-aminopurine and guanine, adenine, cytosine, thymine or hypoxanthine, respectively. Two oligodeoxyribonucleotide duplexes that were site-selectively substituted with a single 2-aminopurine moiety were also studied. A strong quenching of the ...
متن کاملUnusual 2-aminopurine fluorescence from a complex of DNA and the EcoKI methyltransferase.
The methyltransferase, M.EcoKI, recognizes the DNA sequence 5'-AACNNNNNNGTGC-3' and methylates adenine at the underlined positions. DNA methylation has been shown by crystallography to occur via a base flipping mechanism and is believed to be a general mechanism for all methyltransferases. If no structure is available, the fluorescence of 2-aminopurine is often used as a signal for base flippin...
متن کاملTime-resolved fluorescence of 2-aminopurine as a probe of base flipping in M.HhaI–DNA complexes
DNA base flipping is an important mechanism in molecular enzymology, but its study is limited by the lack of an accessible and reliable diagnostic technique. A series of crystalline complexes of a DNA methyltransferase, M.HhaI, and its cognate DNA, in which a fluorescent nucleobase analogue, 2-aminopurine (AP), occupies defined positions with respect the target flipped base, have been prepared ...
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ژورنال
عنوان ژورنال: ChemPhysChem
سال: 2008
ISSN: 1439-4235,1439-7641
DOI: 10.1002/cphc.200890030